MOPS Buffer CAS 1132-61-2
Unilong supplies MOPS free acid as a white powder for commercial buffer and biochemical reagent manufacturing. The commercial specification includes an assay of at least 99.0% on a dried basis, with supply in 25 kg drums. Applications include diagnostic reagents, culture media, protein purification and electrophoresis buffers.
Product Details
| Item | Details |
|---|---|
| Chemical name | 3-(N-Morpholino)propanesulfonic acid |
| CAS number | 1132-61-2 |
| Product form | Free acid, white powder |
| Molecular formula | C7H15NO4S |
| Molecular weight | 209.26 g/mol |
| Commercial packaging | 25 kg/drum |
Buffering and Solution Properties
MOPS is a water-soluble Good's buffer with a useful buffering range of approximately pH 6.5–7.9 and a pKa of approximately 7.2 at 25°C. It provides pH control in aqueous biological formulations operating near neutrality.
The free acid forms an acidic solution before neutralisation. Its specified pH is 2.5–4.5 for a 1% aqueous solution at 25°C. This supply form is distinct from MOPS sodium salt, CAS 71119-22-7.
Technical Specifications
| Test item | Specification | Test condition or basis |
|---|---|---|
| Appearance | White powder | Visual appearance |
| Assay | ≥99.0% | Dried basis |
| pH | 2.5–4.5 | 1% aqueous solution at 25°C |
| Heavy metals | ≤5 ppm | Expressed as Pb |
| Iron | ≤5 ppm | Fe content |
| Loss on drying | ≤0.5% | 105°C |
| pKa | 7.10–7.30 | 20°C |
| Solubility | Passes test | 10% solution in water |
| Solution colour | ≤50 Hazen | 1 M solution |
| UV absorbance | ≤0.02 | 0.1 M aqueous solution, 260 nm, 1 cm path against water |
GMP, sterile, low-endotoxin and nuclease-free status are not claimed for this specification.
Applications
Diagnostic Reagent Manufacturing
MOPS is used as a buffering raw material in selected biochemical diagnostic reagents. It supports near-neutral aqueous reagent systems containing proteins or enzymes. Solution colour and UV absorbance are relevant where the buffer contributes to the optical measurement background. Compatibility remains specific to the assay chemistry and detection wavelength.
Cell Culture and Microbial Media
MOPS is used in culture media for bacteria, yeast and selected mammalian cell systems. Its buffering range supports media formulations operating near neutral pH. It is also used in charcoal yeast extract medium. The appropriate concentration is specific to the organism and medium.
Protein Purification and Chromatography
MOPS is used in protein purification and chromatography buffers operating near neutral pH. It dissolves in water for incorporation into aqueous separation buffers. Its interaction with many metal ions is limited, although stronger interaction with iron can occur. MOPS can interfere with the Folin protein assay when present during protein measurement.
Electrophoresis Buffer Manufacturing
MOPS is a buffer component in denaturing RNA agarose gel electrophoresis. It is also used in selected protein electrophoresis systems. Commercial buffer manufacturers use the free acid in method-specific running buffer formulations. The final buffer composition depends on the nucleic acid or protein separation method.
Processing Characteristics
Near-neutral MOPS buffers can be prepared by neutralising the dissolved free acid, commonly with sodium hydroxide. Autoclaving MOPS solutions can cause yellowing; partial decomposition has also been reported during autoclaving with glucose. Membrane filtration is commonly used for MOPS solutions requiring a sterilisation step.
Packaging and Storage
MOPS commercial supply is offered in 25 kg drums. Keep containers tightly closed in a cool, dry storage area and protect the powder from moisture and contamination. Dry material can compact during storage. Prepared buffer solutions have separate storage requirements determined by their formulation.
Commercial Supply Support from Unilong
For commercial MOPS orders, Unilong supports product documentation, supplier qualification, inspection and export coordination.
Factory Visits and Audit Coordination
We coordinate factory visits, supplier questionnaires and buyer-appointed audits with the relevant manufacturing site. Site access and audit scope are arranged with the manufacturer.
Product Specifications and Batch Documents
We coordinate available product specifications, TDS, SDS and applicable batch COA for the selected MOPS supply. Additional test reports and declarations depend on the grade.
Pre-Shipment Inspection and Approval
Buyer-appointed third-party inspection can be coordinated before dispatch. For orders with agreed pre-shipment approval, the goods are held pending the buyer's approval.
Packaging and Export Coordination
We coordinate shipping marks, commercial invoices, packing lists and transport documents. Packing and loading photos can be included in the agreed shipment records.
Further details are available in Service and Support.
Procurement FAQ
Can you supply against our MOPS specification?
We can check your specification against the available commercial supply.
Is nuclease-free status included in this specification?
RNase- and DNase-free status is not claimed for this specification.
What are the MOQ and current lead time?
MOQ and lead time are confirmed with the quotation for the requested commercial quantity and destination.
Can third-party inspection be arranged before shipment?
Yes, by arrangement. We coordinate the inspection provider with the supplying site.
Ordering from Unilong
Contact Unilong for commercial MOPS free acid supply. Send your required specification, quantity and destination.
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